Click "Run" to clear the graph and start the photon counting.
You can change the number of fluorescent molecules illuminated and the probability that a molecule will become excited (equivalent to increasing the intensity of illuminating light). Increasing these numbers speed up the acquisition but causes a problem that is highlighted in the next applet.
Slow the animation down by dragging the "Graph Update Speed" slider to the left to get a better look at how single photon evens are accumulated in the bar graph. The counter at the bottom shows how many photons have been detected.
If your browser does not have the correct Java support to view this applet. Please visit www.java.com.