Beneke T and Gluenz E (2018) LeishGEdit: a method for rapid gene knockout and tagging using CRISPR-Cas9. Methods in Molecular Biology, in press.


Beneke T, Demay F, Hookway E, Ashman N, Jeffery H, Smith J, Valli J, Becvar T, Myskova J, Lestinova T, Sadlova J, Volf P, Wheeler RJ and Gluenz E (2018). Genetic dissection of the Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections. biorXiv doi:

Costa FC, Francisco AF, Jayawardhana S, Calderano SG, Lewis MD, Olmo F, Beneke T, Gluenz E, Sunter J, Dean S, Kelly JM and Taylor MC (2018) Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping. PLoS Negl Trop Dis. 12(4):e0006388.

Ooi C-P, Smith TK, Gluenz E, Wand NV, Vaughan S, Rudenko G. (2018) Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei. Traffic 1–15.


Martel D, Beneke T, Gluenz E, Späth GF, Rachidi N. (2017) Characterisation of Casein kinase 1.1 in Leishmania donovani using the CRISPR Cas9 toolkit. BioMed Res. Int Article ID 4635605. doi:10.1155/2017/4635605.

Beneke T, Madden R, Makin L, Valli J, Sunter J, Gluenz E. (2017) A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids. R Soc Open Sci. 4(5):170095.


Sanchez MA, Tran KD, Valli J, Hobbs S, Johnson E, Gluenz E, Landfear SM. (2016) KHARON is an essential cytoskeletal protein involved in the trafficking of flagellar membrane proteins and cell division in African trypanosomes. J Biol Chem 291(38):19760-73.


Fiebig M, Kelly S, Gluenz E. (2015) Comparative life cycle transcriptomics revises Leishmania mexicana genome annotation and links a chromosome duplication with parasitism of vertebrates. PLoS Pathogens 11(10):e1005186.

Wheeler RJ, Gluenz E, Gull K. (2015) Routes to a 9+0 flagellum: Basal body multipotency and axonemal plasticity. Nature Commun 6:8964.

Makin L, Gluenz E (2015).
cAMP signalling in trypanosomatids: role in pathogenesis and as a drug target. Trends Parasitol. 31:373-9.

Tran KD, Vieira DP, Sanchez MA, Valli J, Gluenz E, Landfear SM. (2015) Kharon1 null mutants of Leishmania mexicana are avirulent in mice and exhibit a cytokinesis defect within macrophages. PLoS One 10:e0134432.

Gluenz E, Wheeler R J, Hughes L, Vaughan S. (2015) Scanning and three-dimensional electron microscopy methods for the study of Trypanosoma brucei and Leishmania mexicana flagella. Methods Cell Biol. 2015;127:509-42.

Dean S, Sunter J, Wheeler R, Hodkinson I, Gluenz E, Gull K (2015). A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids. Open Biol. 5(1):140197.


Fiebig M, Gluenz E, Carrington M, Kelly S (2014). SLaP mapper: A webserver for identifying and quantifying spliced-leader addition and polyadenylation site usage in kinetoplastid genomes. Mol Biochem Parasitol 196: 71-74.


Wheeler RJ, Gluenz E, Gull K. (2013). The limits on trypanosomatid morphological diversity. PLoS One, 8:e79581.

Povelones ML, Tiengwe C, Gluenz E, Gull K, Englund PT and Jensen RE. (2013). Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1. Mol Microbiol 87, 713-729.


Povelones ML, Gluenz E, Dembek M, Gull K, Rudenko G. (2012). Histone H1 plays a role in heterochromatin formation and VSG expression site silencing in Trypanosoma brucei. PLoS Pathog, 8:e1003010.

Wheeler R., Gull, K and Gluenz, E. (2012). Detailed interrogation of trypanosome cell biology via differential organelle staining and image analysis. BMC Biology, 10:1.


Gluenz, E., Barker, A. R. and Gull, K. (2011). An expanded family of proteins with BPI/LBP/Plunc-like domains in trypanosome parasites: an association with pathogenicity? Biochem Soc Trans 39, 966-70.

Clayton, A. M., Guler, J. L., Povelones, M. L., Gluenz, E., Gull, K. Smith, T. K., Jensen, R. E., and P. T. Englund. (2011). Depletion of mitochondrial acyl carrier protein in bloodstream form Trypanosoma brucei causes a kinetoplast segregation defect. Eukaryotic Cell 10, 286-92.

Gluenz, E.*, Povelones, M. L.*, Englund, P. T. and Gull, K. (2011). The kinetoplast duplication cycle in Trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis. Mol Cell Biol 31, 1012-21.

Wheeler R., Gluenz, E. and Gull, K. (2011). The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology. Mol Microbiol 79, 647-62.


Wickstead, B., J. T. Carrington, E. Gluenz, and K. Gull. (2010). The expanded Kinesin-13 repertoire of trypanosomes contains only one mitotic Kinesin indicating multiple extra-nuclear roles. PLoS One 5:e15020.

Gluenz, E., Höög, J. L., Smith, A. E., Dawe, H. R., Shaw, M. K. and Gull, K. (2010). Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans. FASEB J 24, 3117-21.

Höög, J. L., Gluenz, E., Vaughan, S. and Gull, K. (2010). Ultrastructural investigation methods for Trypanosoma brucei. In Electron Microscopy of Model Systems, vol. 96 (ed. T. Mueller-Reichert), pp. 175-194: Academic Press.
Gluenz, E., Ginger, M. L. and McKean, P. G. (2010). Flagellum assembly and function during the Leishmania life cycle. Curr Opin Microbiol 13, 473-9.
Smith, T. K., Vasileva, N., Gluenz, E., Terry, S., Portman, N., Kramer, S., Carrington, M., Michaeli, S., Gull, K. and Rudenko, G. (2009). Blocking variant surface glycoprotein synthesis in Trypanosoma brucei triggers a general arrest in translation initiation. PLoS One 4, e7532.

Sharma, R., Gluenz, E., Peacock, L., Gibson, W., Gull, K. and Carrington, M. (2009). The heart of darkness: growth and form of Trypanosoma brucei in the tsetse fly. Trends Parasitol 25, 517-24.
Signorell, A.**, Gluenz, E.**, Rettig, J., Schneider, A., Shaw, M. K., Gull, K. and Butikofer, P. (2009). Perturbation of phosphatidylethanolamine synthesis affects mitochondrial morphology and cell-cycle progression in procyclic-form Trypanosoma brucei. Mol Microbiol 72, 1068-79.

Lindsay, M. E.*, Gluenz, E.*, Gull, K. and Englund, P. T. (2008). A new function of Trypanosoma brucei mitochondrial topoisomerase II is to maintain kinetoplast DNA network topology. Mol Microbiol 70, 1465-76.
Barker, A. R., Wickstead, B., Gluenz, E. and Gull, K. (2008). Bioinformatic insights to the ESAG5 and GRESAG5 gene families in kinetoplastid parasites. Mol Biochem Parasitol 162, 112-22.
Gluenz, E., Sharma, R., Carrington, M. and Gull, K. (2008). Functional characterization of cohesin subunit SCC1 in Trypanosoma brucei and dissection of mutant phenotypes in two life cycle stages. Mol Microbiol 69,
Sharma, R., Peacock, L., Gluenz, E., Gull, K., Gibson, W. and Carrington, M. (2008). Asymmetric cell division as a route to reduction in cell length and change in cell morphology in trypanosomes. Protist 159, 137-51.

Gluenz, E., Shaw, M. K. and Gull, K. (2007). Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast. Mol Microbiol 64, 1529-39.
Gluenz, E., Taylor, M. C. and Kelly, J. M. (2007). The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements. Int J Parasitol 37, 617-25.






Joining the Lab

egluenz, 24/04/2018