Phenotypic drug discovery is seeing a resurgence in popularity but its main disadvantage is that the drug target is unknown which leads to a blind medicinal chemistry campaign and difficulties monitoring clinical success. Thus, target deconvolution
is of increasing significance as a means of improving attrition in the drug discovery process. My research has aimed to identify the targets of the small molecule ezutromid for the treatment of Duchenne Muscular Dystrophy. Duchenne muscular
dystrophy (DMD) is an X-linked muscle wasting disease which affects about 1 in 5000 boys. DMD leads to loss of ambulation and reduction of average life expectancy to the late 20s, and there is currently no cure. It is caused by loss of dystrophin
protein, from one of hundreds of different dystrophin gene mutations.
January - March 2020
Russell and Milne labs/OxStem Oncology
Molecular mechanism discovery for small molecule inducers of myeloblast differentiation for AML
October - December 2019
Worked for the International Commission on the Clinical Use of Human Germline Genome Editing
October 2015 - October 2019
Elucidation of the mechanism of action of utrophin modulators for the treatment of Duchenne Muscular Dystrophy
July 2016 to October 2016
In silico and in vitro studies towards identification of chemical probes targeting BPTF BRD and PHD2 domains.
October 2014 to Jun 2015
Paterson lab Investigation of novel desymmetrising aldol reactions on meso-dialdehydes
June 2014 to September 2014
Harrowven lab Preparation of precursors for the Total Synthesis of RP-66453 (natural product).
June 2013 to August 2013
Gurdon Institute, Miska lab Investigating antiviral immunity in C. elegans by forward genetic screen.
Easter and September 2013
Callaghan lab Characterisation of sites on the E. coli small non-coding RNA MicA involved in Hfq chaperone binding.
October 2015 to present
Department of Chemistry, University of Oxford
October 2011 to 2015
University of Cambridge, Christ's College
Specialising in Chemistry, further modules taken in Biochemistry, Cell Biology and Physiology
|Design and synthesis of chemical probes with photoaffinity (diazirine) and biotin tags, Structure activity relationship studies, stereoselective synthesis, mg to multi gram scale
|Biochemistry:||Affinity chromatography for target ID, CETSA, Western blot, RT-qPCR, enzyme inhibition assays, luciferase reporter assays, thermal shift assays, protein purification, cloning
|Proteomics:|| Sample preparation, LC-MS/MS submission, data analysis (Maxquant)
|Structural Biology:|| Protein crystallisation, structure solution by molecular replacement
|Cell biology:||Tissue culture, microscopy
|Isabel V. L. Wilkinson et al. Chemical Proteomics and Phenotypic Profiling Identifies the Aryl Hydrocarbon Receptor as a Molecular Target of the Utrophin Modulator Ezutromid, Angewandte Chemie, Nov 2019.|
|Isabel V. L. Wilkinson et al. Characterisation of utrophin modulator SMT C1100 as a non-competitive inhibitor of firefly luciferase, Bioorganic chemistry, Oct 2019.|
|Maria Chatzopoulou et al. (alphabetical author order including Isabel V. L. Wilkinson) Isolation, Structural Identification, Synthesis, and Pharmacological Profiling of 1,2-trans-Dihydro-1,2-diol Metabolites of the Utrophin Modulator Ezutromid, J. Med. Chem., Oct 2019.|
|Oral Presentation, Spring 2019 ACS National Meeting, Orlando (2019).|
|Oral Presentation, ABPP 2019 - Target Discovery and Visualisation using chemical probes, Leuven, Belgium (2019).|
|STEM for Britain 2019, House of Commons poster presentation, March 2019|
Teaching the Organic Chemistry course examined in the First Public Examination to Biochemistry undergraduate students.
Demonstrator, Doctoral Training Centre, University of Oxford Introduction to Programming and Drug Discovery modules, which are intensive two-week courses to introduce interdisciplinary students to unfamiliar fields.
|Pfizer symposium poster prize
|2015-2019||Pembroke College graduate scholarship
|2014|| Sir Ian MacFarlane prize for Excellence (£1000) and the Todd Prize (Christ's College)
|2013|| Grieg Prize for Natural Science (Christ's College)
|STEM Ambassador and six years of volunteering with both Oxford and Cambridge Hands-on Science, bringing small scale scientific demonstrations to schools and the public around the country.
Successful application to the Royal Society of Chemistry Outreach Fund 2018 (£2000)
|Enterprise||Medicinal Chemistry lead for the start-up Spherox, participated in the 2018 PanaceaStars accelerator and bootcamp.
|Badminton|| 2016-2017 Captain of the Pembroke College badminton team.
2010-2011 Captain of the Christ's College women's badminton team.
|Languages|| Mandarin Chinese and intermediate German
|Not for profit||Fundraising Officer then Treasurer for Save the Children university society 2012-2014, winning a prize in the Save the Children ultimate uni challenge.