Welcome to Stephan Uphoff's research site
I am a postdoctoral researcher studying bacterial chromosome biology using single-molecule and single-cell microscopy. Explore this website to find out more about my research interests, projects, and publications.
16th June 2016: Book chapter in Springer Protocols
Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time.
I have contributed a chapter entitled "Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells" to this volume in the Methods in Molecular Biology series. It features a step-by-step, readily reproducible laboratory protocol with a list of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
6th June 2016: New publication evaluates different super-resolution microscopy techniques
Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison has been published in Scientific Reports, in collaboration with Micron Advanced Microscopy Unit, Oxford. In this study, popular super-resolution microscopy techniques are tested side-by-side, revealing their individual strengths and weaknesses.
4th March 2016: Paper on cell-to-cell variation in mutagenesis is out
Stochastic activation of a DNA damage response causes cell-to-cell mutation rate variation appeared in Science. This project, a collaboration with Johan Paulsson's lab at Harvard Medical School, showed that success or failure to induce a DNA damage response is dictated by the stochastic presence or absence of a single protein molecule per cell, which causes mutation rate variation between distinct cell subpopulations.