Welcome to Stephan Uphoff's research site
I am a postdoctoral researcher studying bacterial chromosome biology using single-molecule and single-cell microscopy. Explore this website to find out more about my research interests, projects, and publications.
16th June 2016: Book chapter in Springer Protocols
Chromosome Architecture: Methods and Protocols guides readers through cutting-edge interdisciplinary methods which allow for an understanding of architecture of chromosomes with exceptionally enhanced resolution, both in terms of space and time.
I have contributed a chapter entitled "Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells" to this volume in the Methods in Molecular Biology series. It features a step-by-step, readily reproducible laboratory protocol with a list of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
6th June 2016: New publication evaluates different super-resolution microscopy techniques
Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison has been published in Scientific Reports, in collaboration with Micron Advanced Microscopy Unit, Oxford. In this study, popular super-resolution microscopy techniques are tested side-by-side, revealing their individual strengths and weaknesses.
31st August 2016: A new model for the initiation of bacterial Nucleotide Excision Repair
Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli appeared in Nature Communications. This project revealed distinct roles of UvrA and UvrB proteins in the search and verification of DNA lesions.