Welcome to the Uphoff lab
Department of Biochemistry | University of Oxford
Our group investigates DNA repair and mutagenesis in bacteria. We seek to understand the mechanisms of DNA repair pathways and how mutation rates are regulated through DNA damage responses. Our goal is to directly measure these fundamental processes inside individual living cells. To this end, we develop single-molecule/super-resolution microscopy, single-cell manipulation, microfluidics, flow cytometry, and whole genome sequencing techniques. We also advance data analysis and computational modeling for quantitative descriptions of DNA repair and mutagenesis. We are currently very interested in the consequences of gene expression noise and stochastic processes on the fidelity of genome maintenance. This work also informs about the origins of mutations that lead to antibiotic resistance.
Join our group for your PhD
Sir Henry Dale Fellowship
Stephan has been awarded a Sir Henry Dale Fellowship by the Wellcome Trust to set up a research group at the Department of Biochemistry, University of Oxford.
Scc2/Nipbl Hops Between Chromosomal Cohesin Rings After Loading
Preprint article from our collaboration with James Rhodes and Kim Nasmyth now on bioRxiv.
Annual Reviews of Biophysics
Our article Single-molecule analysis of bacterial DNA repair and mutagenesis is now published in the Annual Reviews of Biophysics.
Frequent exchange of the DNA polymerase during bacterial chromosome replication
New publication in Elife from our collaboration with Rodrigo Reyes-Lamothe's lab.
New model for DNA damage recognition
Our article Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli proposes a new model for the initiation of nucleotide excision repair.