Bacterial DNA Repair and Mutagenesis


Uphoff Lab | Department of Biochemistry | University of Oxford

Our group investigates DNA repair and mutagenesis in bacteria. We seek to understand the mechanisms of DNA repair pathways and how mutation rates are regulated through DNA damage responses. Our goal is to directly measure these fundamental processes inside individual living cells. To this end, we develop single-molecule/super-resolution microscopy, single-cell manipulation, microfluidics, flow cytometry, and whole genome sequencing techniques. We also advance data analysis and computational modeling for quantitative descriptions of DNA repair and mutagenesis. We are currently very interested in the consequences of gene expression noise and stochastic processes on the fidelity of genome maintenance. This work also informs about the origins of mutations that lead to antibiotic resistance.


Upcoming conferences and seminar talks

School of Biological Sciences, University of Edinburgh. Departmental Seminar Series. 6th November 2017.

Poznan University. 5th February 2018.

DNA and Interacting Proteins as Single Molecules - In Vitro and In Vivo Conference. Cancun. 21st-24th February 2018.

Microbiology Society Annual Conference. Birmingham. 10th-13th April 2018.

European Light Microscopy Initiative (ELMI) Conference. Dublin. 4th-8th June 2018.

Single Molecule Bacteriology Biochemical Society Harden Conference. Oxford. 9th-12th September 2018.

The Uphoff lab is growing!

December 2017

Welcome to Valentine Lagage who joined the lab as a postdoc and Nehir Banaz who is a new D.Phil. student.


The New Microscopy

November 2017

Special Edition of Current Opinion in Microbiology co-edited with Ariane Briegel. Watch out for more articles appearing in the coming months.


Welcome to Emma Jones

September 2017

Emma is joining the lab for her 4th year research project.


Wellcome-Beit Prize

September 2017

Stephan has won a Wellcome-Beit Prize. A very welcome boost of funding for the new lab!

Join our group

September 2017

Students interested in joining our lab for their PhD can apply to different graduate programs at Oxford University. Find out more and contact me directly.


Sir Henry Dale Fellowship

March 2017

Stephan has been awarded a Sir Henry Dale Fellowship by the Wellcome Trust to set up a research group at the Department of Biochemistry, University of Oxford.


Scc2/Nipbl Hops Between Chromosomal Cohesin Rings After Loading

September 2017

Live-cell single-molecule tracking of the cohesin loader protein. This article from our collaboration with James Rhodes and Kim Nasmyth is now published in eLife.