Publications

J. J. Tao, P. Castel, N. Radosevic-Robin, M. Elkabets, N. Auricchio, N. Aceto, G. Weitsman, P. Barber, B. Vojnovic, H. Ellis, N. Morse, N. T. Viola-Villegas, A. Bosch, D. Juric, S. Hazra, S. Singh, P. Kim, A. Bergamaschi, S. Maheswaran, T. Ng, F. Penault-Llorca, J. S. Lewis, L. A. Carey, C. M. Perou, J. Baselga, and M. Scaltriti, Antagonism of EGFR and HER3 Enhances the Response to Inhibitors of the PI3K-Akt Pathway in Triple-Negative Breast Cancer, Sci. Signal. 7, ra29 (2014).

P. R. Barber, I. D. C. Tullis, M. I. Rowley, C. D. Martins, G. Weitsman, K. Lawler, M. Coffey, N. Woodman, C. E. Gillett, T. Ng, and B. Vojnovic, The Gray Institute open microscopes applied to radiobiology and protein interaction studies, Proc. SPIE 8949, 89490D-89490D-17 (2014).

P.A. Young, A. Grislis, P.R. Barber, P.J. Keely, K.W. Eliceiri. Data Processing for Time-Domain Fluorescence Lifetime Imaging Microscopy. Microscopy and Microanalysis 19: 758-759 doi:10.1017/S1431927613005783 (2013).

P. R. Barber, I. D. C. Tullis, G. P. Pierce, R. G. Newman, J. Prentice, M. I. Rowley, D. R. Matthews, S. M. Ameer-Beg, and B. Vojnovic. The Gray Institute 'Open' High-content, Fluorescence Lifetime Microscopes. J. Microscopy. doi:10.1111/jmi.12057 (2013). Supporting Information

J. C. G. Jeynes, M. J. Merchant, L. Barazzuol, M. Barry, D. Guest, V. V. Palitsin, G. W. Grime, I. D. C. Tullis, P. R. Barber, B. Vojnovic, and K. J. Kirkby, "Broadbeam" irradiation of mammalian cells using a vertical microbeam facility, Radiat Environ Biophys 52, 513-521 (2013).

S. Akerman, M. Fisher, R. A. Daniel, D. Lefley, C. C. Reyes-Aldasoro, S. J. Lunt, S. Harris, M. Bjorndahl, L. J. Williams, H. Evans, P. R. Barber, B. Vojnovic, C. Kanthou, G. M. Tozer, Influence of Soluble or Matrix-bound Isoforms of Vascular Endothelial Growth factor-A on Tumor Response to Vascular-targeted Strategies. Int. J. Cancer. doi:10.1002/ijc.28281 (2013).

M. J. Merchant, J. C. G. Jeynes, G. W. Grime, V. Palitsin, I. D. C. Tullis, P. R. Barber, B. Vojnovic, R. P. Webb, and K. J. Kirkby, A focused scanning vertical beam for charged particle irradiation of living cells with single counted particles. Radiation Research 178, 182-190 (2012).

H. C. Mandeville, Q. S. Ng, F. M. Daley, P. R. Barber, G. Pierce, J. Finch, M. Burke, Mb. Bell, E. R. Townsend, R. Kozarski, B. Vojnovic, P. J. Hoskin, and V. Goh, Operable Non-Small Cell Lung Cancer: Correlation of Volumetric Helical Dynamic Contrast-Enhanced CT Parameters with Immunohistochemical Markers of Tumor Hypoxia. Radiology (2012).

D. R. Matthews, G. O. Fruhwirth, G. Weitsman, L. M. Carlin, E. Ofo, M. Keppler, P. R. Barber, I. D. C. Tullis, B. Vojnovic, T. Ng, and S. M. Ameer-Beg, A Multi-Functional Imaging Approach to High-Content Protein Interaction Screening, PLoS ONE 7, e33231 (2012).

K. Rothkamm, J. C. Crosbie, F. Daley, S. Bourne, P. R. Barber, B. Vojnovic, L. Cann, and P. A. W. Rogers, In situ Biological Dose Mapping Estimates the Radiation Burden Delivered to "Spared" Tissue between Synchrotron X-Ray Microbeam Radiotherapy Tracks, PLoS ONE 7, e29853 (2012).

M. Baker, S. D. Robinson, T. Lechertier, P. R. Barber, B. Tavora, G. D'Amico, D. T. Jones, B. Vojnovic, and K. Hodivala-Dilke, Use of the mouse aortic ring assay to study angiogenesis, Nat. Protocols 7, 89-104 (2012).

L. M. Carlin, R. Evans, H. Milewicz, L. Fernandes, D. R. Matthews, M. Perani, J. Levitt, M. D. Keppler, J. Monypenny, T. Coolen, P. R. Barber, B. Vojnovic, K. Suhling, F. Fraternali, S. Ameer-Beg, P. J. Parker, N. S. B. Thomas, and T. Ng, A Targeted siRNA Screen Identifies Regulators of Cdc42 Activity at the Natural Killer Cell Immunological Synapse, Sci. Signal. 4, ra81 (2011).

S. E. Rademakers, P. F. Rijken, W. J. Peeters, M. M. Nijkamp, P. R. Barber, J. Laak, A. J. Kogel, J. Bussink, and J. H. Kaanders, Parametric mapping of immunohistochemically stained tissue sections; a method to quantify the colocalization of tumor markers, Cellular Oncology 34, 119-129 (2011).

I. A. Pope, P. R. Barber, S. Horn, E. Ainsbury, K. Rothkamm, and B. Vojnovic, A portable microfluidic fluorescence spectrometer device for [gamma]-H2AX-based biological dosimetry, Radiation Measurements 46, 907-911 (2011).

B. Cornelissen, S. Darbar, R. Hernandez, V. Kersemans, I. Tullis, P. R. Barber, S. Smart, B. Vojnovic, R. Reilly, and K. A. Vallis, ErbB-2 Blockade and Prenyltransferase Inhibition Alter Epidermal Growth Factor and Epidermal Growth Factor Receptor Trafficking and Enhance 111In-DTPA-hEGF Auger Electron Radiation Therapy, Journal of Nuclear Medicine 52, 776 -783 (2011).

G. S. Patel, T. Kiuchi, K. Lawler, E. Ofo, G. O. Fruhwirth, M. Kelleher, E. Shamil, R. Zhang, P. R. Selvin, G. Santis, J. Spicer, N. Woodman, C. E. Gillett, P. R. Barber, B. Vojnovic, G. Keri, T. Schaeffter, V. Goh, M. J. O'Doherty, P. A. Ellis, and T. Ng, The challenges of integrating molecular imaging into the optimization of cancer therapy, Integr. Biol. (2011).

Fruhwirth G. O., et al., How Forster Resonance Energy Transfer Imaging Improves the Understanding of Protein Interaction Networks in Cancer Biology. ChemPhysChem, 2011. 12, 442-461.

Rowley M., et al., Bayesian analysis of Fluorescence lifetime imaging data. Proc SPIE, 2011. 7903, p. 74

Barber P.R., et al., A Bayesian method for single molecule, fluorescence burst analysis. Biomed. Opt. Express, 2010 1, p. 1148-1158.

Matthews D.R., et al., Time-lapse FRET microscopy using fluorescence anisotropy, Journal of Microscopy, 2010 237, p. 51-62.

Makrogianneli, K., et al., Integrating Receptor Signal Inputs That Influence Small Rho GTPase Activation Dynamics at the Immunological Synapse. Mol. Cell. Biol., 2009. 29(11): p. 2997-3006.

Barber, P.R., et al., Multiphoton time-domain FLIM: Practical application to protein-protein interactions using global analysis. J R Soc Interface, 2009. 6: p. S93-S105.

Kelleher, M., et al., The potential of optical proteomic technologies to individualize prognosis and guide rational treatment for cancer patients. Targeted Oncology 4, 235-252 (2009).

Badie, S., et al., RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation. J. Cell Biol., 2009. 185(4): p. 587-600.

Vojnovic, B., et al., Detecting Objects, WIPO, Patent:PCT/GB2008/000216, 2008.

Tozer, G.M., et al., Blood Vessel Maturation and Response to Vascular-Disrupting Therapy in Single Vascular Endothelial Growth Factor-A Isoform-Producing Tumors. Cancer Res, 2008. 68(7): p. 2301-2311.

Matthews, D.R., et al., A high-content screening platform utilizing polarization anisotropy and FLIM microscopy. Proc. SPIE, 2008. 6859.

Levitt, J.A., et al., Multidimensional multiphoton fluorescence lifetime imaging of cells. Proc. SPIE, 2008. 6860.

Errington, R.J., et al. Detection Of Gamma-H2ax Foci Using High Content Screening Formats. in ISAC XXIV. 2008. Budapest, Hungary.

Barber, P.R., et al. Towards high-throughput FLIM for protein-protein interaction screening of live cells and tissue microarrays. in 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI '08). 2008.

Reyes-Aldasoro, C.C., et al., Estimation of Apparent Tumor Vascular Permeability from Multiphoton Fluorescence Microscopic Images of P22 Rat Sarcomas In Vivo. Microcirculation, 2007. 15(1): p. 65-79.

Prag, S., et al., Activated Ezrin Promotes Cell Migration through Recruitment of the GEF Dbl to Lipid Rafts and Preferential Downstream Activation of Cdc42. Mol. Biol. Cell, 2007. 18(8): p. 2935-2948.

Pope, I.A., et al., A complete, self-contained, optical biochip with live-cell imaging capability, in SPIE Photonics West. 2007, SPIE: San Jose, USA.

McConnell, G., et al., Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source. Journal of Microscopy, 2007. 225(2): p. 126-136.

Matthews, D.R., et al., A fluorescence biochip with a plasmon active surface. Proc. SPIE, 2007. 6450.

Everett, S.A., et al. Cytochrome P450 CYP1B1 over-expression in malignant melanoma and disseminated disease: identification of a key indication for the enzyme/prodrug therapy of cancer. in AACR Annual Meeting 2007. 2007. Los Angeles, California, USA.

Everett, S.A., et al., Profiling cytochrome P450 CYP1 enzyme expression in primary melanoma and disseminated disease utilizing spectral imaging microscopy (SIM). J Clin Oncol (Meeting Abstracts), 2007. 25(18_suppl): p. 8556-.

Barber, P.R., et al., Gamma-H2AX Foci Counting: Image processing and control software for high-content screening. Proc. SPIE, 2007. 6441: p. 64411M.

Tullis, I.D.C., et al., Mapping femtosecond pulse front distortion and group velocity dispersion in multiphoton microscopy. Proc. SPIE, 2006. 6089: p. 158-164.

Reyes-Aldasoro, C.C., et al., Multiphoton fluorescence microscopy for assessing vascular permeability in experimental tumours; effects of CA-4-P, VEGF isoforms & ANG-1. Angiogenesis, 2006. 9(1): p. 26.

Matthews, D.R., et al., Technique for measurement of fluorescence lifetime by use of stroboscopic excitation and continuous-wave detection. Applied Optics, 2006. 45: p. 2115-2123.

Lucas, L.A.G., et al., Effect Of Angiostatin4.5 On Tumour Blood Vessels. Angiogenesis, 2006. 9(1): p. 38.

Barber, P.R., et al., The GCI Multitarget X-ray Microprobe: Imaging, Control and Software Automation. Extended Abstracts of the 7th International Workshop: Microbeam Probes of Cellular Radiation Response published in Radiation Research, 2006. 166: p. 663-4.

Akerman, S., et al., Tumour Cells Expressing Only Single Vegf Isoforms (120, 164 Or 188) Form Tumours With Very Different Vascular Maturities And Response To Combretastatin A-4-P. Angiogenesis, 2006. 9(1): p. 28.

Tozer, G.M., et al. Tumour susceptibility to the vascular disrupting agent, combretastatin A-4-P, is modified by differential expression of individual isoforms of VEGF-A. in Tumour Microenvironment. 2005. Oxford.

Tozer, G.M., et al. The influence of different isoforms of vascular endothelial growth factor A (VEGF-A) on tumor vascular maturation and response to the vascular disrupting agent, combretastatin A-4 phosphate. in 96th AACR Annual Meeting. 2005. Anaheim CA.

Tozer, G.M., et al., Intravital imaging of tumour vascular networks using multi-photon fluorescence microscopy. Advanced Drug Delivery Reviews, 2005. 57(1): p. 135-152.

Rankov, V., et al., An algorithm for image stitching and blending. Proc. SPIE, 2005. 5701: p. 190-199.

Parsons, M., et al., Spatially Distinct Binding of Cdc42 to PAK1 and N-WASP in Breast Carcinoma Cells. Molecular and Cellular Biology, 2005. 25(5): p. 1680-1695.

Lucas, L.A.G., et al., Molecular targeting of angiogenesis - an intravital imaging study, in NCRI. 2005: Birmingham, UK.

Lucas, L., et al., Combretastatin a-4-p treatment reduces red blood cell velocity in tumour microvessels in a time and dose dependent manner, in Cancer RRG Northern Ireland - annual retreat. 2005: Antrim, Northern Ireland.

Barber, P.R., R.J. Edens, and B. Vojnovic, Acquisition and analysis of spectral image data by linear un-mixing, cluster computing and a novel spectral imager. Proc. SPIE, 2005. 5694: p. 53-61.

Barber, P.R., et al., Global and pixel kinetic data analysis for FRET detection by multi-photon time-domain FLIM. Proc. SPIE, 2005. 5700: p. 171-181.

Barber, P.R. Image Processing and Analysis in 2D and 3D Microscopy. in Optoelectronics for Use in the Diagnosis of Cancer. 2005. Grasmere, Cumbria, UK: The Rank Prize Funds.

Barber, P.R. Multiphoton and Spectral imaging in Cancer Research. in Lecture to MRes students. 2005. King's College London, The Strand, London, UK.

Atkin, G., et al., Correlation of spectral imaging and visual grading for the quantification of thymidylate synthase protein expression in rectal cancer. Hum Pathol, 2005. 36(12): p. 1302-8.

Ameer-Beg, S.M., et al., Dynamic imaging of protein-protein interactions by MP-FLIM. Proc. SPIE, 2005. 5700: p. 152-161.

Wilson, I., et al. 3-Dimensional Characterisation Of Tumour Vascular Networks Using Intravital Multiphoton Fluorescence Microscopy. in Microcirculation. 2004. University of Sheffield, Sheffield, U.K.: British Microcirculation Society.

Vojnovic, B., et al., Cell Imaging Techniques for Use with Microbeams. Extended Abstracts of the 6th International Workshop: Microbeam Probes of Cellular Radiation Response published in Radiation Research, 2004. 161: p. 87-119.

Lucas, L., et al. Intravital Investigation Of The Effects Of An Antivascular And An Anti-Angiogenic Agent On Tumour Microvasculature. in Microcirculation. 2004. University of Sheffield, Sheffield, U.K.: British Microcirculation Society.

Ireson, C.R., et al. Tumour Vascular Maturation And Response To Tubulin-Binding Vascular Targeting Agents. in Microcirculation. 2004. University of Sheffield, Sheffield, U.K.: British Microcirculation Society.

Carnell, D.M., et al., Target validation of cytochrome p450 cyp1b1 in prostate carcinoma and protein expression in associated hyperplastic and pre-malignant tissue. International Journal of Radiation Oncology, Biology and Physics, 2004. 58(2): p. 500-509.

Barber, P.R. Multiphoton and Spectral imaging in Cancer Research. in CBC Colloquium: Molecules to Medicines: Modern Cancer Drug Development. 2004. Chester Beatty Labs, Institute of Cancer Research, SW3 6JB, London, UK.

Greer, M.L., et al., Cytochrome P450CYP1B1 versus GLUT 1 protein expression during the development of head and neck squamous cell carcinomas (HNSCCs). British Journal of Cancer, 2003. 88: p. S33-S33.

Brooks, A.C., et al., The vascular targeting agent combretastatin A-4-phosphate induces neutrophil recruitment to endothelial cells in vitro. Anticancer Res., 2003. 23(4): p. 3199-206.

Barber, P.R., et al., Applications of cost-effective spectral imaging microscopy in cancer research. J. Phys. D: Appl. Phys., 2003. 36(14): p. 17291738.

Barber, P.R., et al., Semi-automated software for the three-dimensional delineation of complex vascular networks. Journal of Microscopy, 2003. 211(1): p. 54-62.

Barber, P.R., et al., Three-dimensional imaging and quantification of complex vascular networks. Proc. SPIE, 2003. 5139: p. 67-78.

Ameer-Beg, S.M., et al., Imaging protein-protein interactions by multiphoton FLIM. Proc. SPIE, 2003. 5139: p. 180-189.

Ameer-Beg, S.M., et al., Application Of Multiphoton steady state and lifetime imaging to mapping of tumour vascular architecture in vivo. Proc. SPIE 4620, 2002: p. 85-95.

Ameer-Beg, S., et al. Multiphoton Steady-state and Lifetime Imaging of Tumour Vascular Architecture 'in Vivo'. in Photon'02. 2002. Cardiff, UK.

Tozer, G.M., et al., Mechanisms Associated with Tumor Vascular Shut-Down Induced by Combretastatin A-4 Phosphate: Intravital Microscopy and Measurement of Vascular Permeability. Cancer Research, 2001. 61(September 1): p. 6413-6422.

Barber, P.R., et al., Automated Counting of Mammalian Cell Colonies. Physics in Medicine and Biology, 2001. 46(1): p. 63-76.

Maxwell, R.J., et al. Segmentation of magnetic resonance images according to contrast agent uptake kinetics using a competitive neural network. in Artificial Neural Networks in Medicine and Biology (ANNIMAB-1). 2000. Goteborg, Sweden: Springer, London.

Loukas, C.G., et al. An edge detection approach for automated counting of cancer cell nuclei in large-scale histological images. in Proceedings of Medical Image Understanding and Analysis, MIUA2000. 2000. London, UK: UCL, London.

Barber, P.R., et al. An automated colony counter utilising a compact Hough transform. in Medical Image Understanding and Analysis, MIUA2000. 2000. London, UK: Communications in Print Plc, UCL.

Paschotta, R., et al., Lifetime quenching in Yb-doped fibres. Optics Communications, 1997. 136: p. 375-378.

Paschotta, R., et al., Characterization and modeling of thulium:ZBLAN blue upconversion fiber lasers. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 1997. 14(5): p. 1213-1218.

Basten, M., et al. Sensor combinations for urban roads and expressways (SECURE). in Proceedings of Automotive Vehicle Technologies, Autotech '97. 1997. Birmingham, UK: Mechanical Engineering Publications for The Institution of Mechanical Engineers, Bury St Edmunds.

Pask, H.M., et al., Ytterbium-doped silica fibre lasers: versatile sources for the 1-1.2mm region. IEEE Journal on Selected Topics in Quantum Electronics, 1995. 1(1): p. 2-13.

Barber, P.R., et al., Infrared-induced photodarkening in Tm-doped fluoride fibres. Optics Letters, 1995. 20(21): p. 2195.

Pask, H.M., et al. Upconversion laser action in Pr3+-doped fluoride fibre pumped by an Yb-doped silica fibre laser. in Advanced Solid-State Lasers, Technical Digest. 1994: Optical Society of America, Washington, DC.

Pask, H.M., et al. Recent advances in Yb3+-doped silica fibre lasers. in CLEO '94 Conference on lasers and electro-optics, Technical Digest. 1994. Anaheim, CA, USA: Optical Society of America, Washington, DC.

Barber, P.R., et al. Improved laser performance of Tm3+ and Pr3+-doped ZBLAN fibres. in 1994 Conf. on Lasers and Electro-Optics Europe, Technical Digest. 1994. Amsterdam, The Netherlands: IEEE.

Barber, P.R., et al. Efficient up conversion laser action in Tm3+ and Pr3+-doped ZBLAN fibres. in CLEO '94 Conference on lasers and electro-optics, Technical Digest. 1994. Anaheim, CA, USA: Optical Society of America, Washington, DC.

Barber, P.R., et al. All solid state blue room-temperature thulium-doped up conversion fibre laser. in Compact Blue-Green Lasers, 1994 Technical Digest Series. 1994. Salt Lake City, USA: Optical Society of America, Washington, DC.

Mackechnie, C.J., et al. Ytterbium fibre lasers: versatile sources for the 1-1.2 mm region. in CLEO '93 Conference on lasers and electro-optics, Technical Digest. 1993. Baltimore, USA: Optical Society of America, Washington, DC.