This medium is suitable for growing transfectants using Glutamine Synthetase selection (CellTech Vectors) or, after the addition of glutamine, for growing most hybridomas, including those derived from NS0 and Y3.
Component Concentration (1x) Concentration (stock)
(mg/L) (mg/L)
INORGANIC SALTS:CaCl2 (anhyd.) 165.02 KCl 330.00 KNO3 0.076 MgSO4 (anyhd.) 97.67 NaCl 5660.00 NaH2PO4.H2O 125.00 Na2SeO3.5H2O 0.017
AMINO ACIDS: 1x 50xL-Alanine 25.00 1250 L-Asparagine (free base) 325.00 16250 L-Arginine.HCl 84.00 4200 L-Aspartic Acid 30.00 1500 L-Cystine.2HCl 91.24 4562 L-Glutamic Acid 375.00 18750 Glycine 30.00 1500 L-Histidine HCl.H2O 42.00 2100 L-Isoleucine 105.00 5250 L-Leucine 105.00 5250 L-Lysine.HCl 146.00 7300 L-Methionine 30.00 1500 L-Phenylalanine 66.00 3300 L-Proline 40.00 2000 L-Serine 42.00 2100 L-Threonine 95.00 4750 L-Tryptophan 16.00 800 L-Tyrosine (disodium).2H2O 104.20 5210 L-Valine 94.00 4700
VITAMINS: 1x 100xBiotin 0.013 1.3 D-Ca Pantothenate 4.00 400 Choline Chloride 4.00 400 Folic Acid 4.00 400 i-Inositol 7.20 720 Nicotinamide 4.00 400 Pyridoxal HCl 4.00 400 Riboflavin 0.40 40 Thiamine HCl 4.00 400 Vitamin B12 0.013 1.3
RIBONUCLEOSIDES: 1x 100xAdenosine 7.00 700 Cytidine 7.00 700 Guanosine 7.00 700 Uridine 7.00 700 Thymidine 2.40 240
OTHER COMPONENTS:D-Glucose 4500.00 Phenol Red 15.00 Sodium Pyruvate 110.00 2-Mercaptoethanol 3.5 microlitres/litre
NaHCO3 3700.00
HCl to final pH7.1 (approx 1.5ml/L of 5M)