Monovalent streptavidin protein (J Mol Biol 2013 and Nature Methods 2006) is available for the academic community as a resource from our lab. There is no need to collaborate and no price charged for the materials, but you will need to pay for shipping. Please e-mail Mark with a very brief description of the application and why you expect that regular streptavidin will not suffice. Traptavidin is available here.
The following plasmids we have given to the plasmid repository Addgene for easy distribution to academic laboratories. For those in the UK, we can send plasmids to you directly if you prefer. Non-academics should contact Mark or the Oxford University technology transfer office, Isis Innovation. Feel free to e-mail for comments or advice.
Traptavidin, a mutant of streptavidin with lower off-rate, increased thermostability, and greater mechanical strength.
SpyTag-MBP for irreversible peptide labelling
SpyCatcher for irreversible peptide labelling
SpyCatcher EQ as a non-reactive control for SpyCatcher
Copyright © 2014 Mark Howarth. All rights reserved.