Mark Howarth lab

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Reagent distribution

Protein

Monovalent streptavidin protein (J Mol Biol 2013 and Nature Methods 2006) is available for the academic community as a resource from our lab. There is no need to collaborate and no price charged for the materials, but you will need to pay for shipping. Please e-mail Mark with a very brief description of the application and why you expect that regular streptavidin will not suffice. Traptavidin is available here.

Plasmids

The following plasmids we have given to the plasmid repository Addgene for easy distribution to academic laboratories. For those in the UK, we can send plasmids to you directly if you prefer. Non-academics should contact Mark or the Oxford University technology transfer office, Isis Innovation. Feel free to e-mail for comments or advice.

Traptavidin, a mutant of streptavidin with lower off-rate, increased thermostability, and greater mechanical strength.

Monovalent streptavidin and Divalent streptavidin: Alive-H6 or Alive-E6 and Dead or Dead-Aspartate loop subunits (J Mol Biol 2013 and Nature Methods 2006)

SpyTag-MBP for irreversible peptide labelling

SpyCatcher for irreversible peptide labelling

SpyCatcher EQ as a non-reactive control for SpyCatcher

 Copyright 2014 Mark Howarth. All rights reserved.