Publications
Publications
2013
Hua, H, M. Namdar, O. Ganier, J. Gregan, M Mechali, and S.E. Kearsey. 2013. Sequential steps in DNA replication are inhibited to ensure reduction of ploidy in meiosis. Molecular Biology of the Cell in press.
2012
Salguero, I., E. Guarino, M.E. Shepherd, T.D. Deegan, C.G. Havens, S.A. MacNeill, J.C. Walter, and S.E. Kearsey. 2012. Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen. Curr Biol. 22:720-726.
Palles, C., J.B. Cazier, K.M. Howarth, E. Domingo, A.M. Jones, P. Broderick, Z. Kemp, S.L. Spain, E.G. Almeida, I. Salguero, A. Sherborne, D. Chubb, L.G. Carvajal-Carmona, Y. Ma, K. Kaur, S. Dobbins, E. Barclay, M. Gorman, L. Martin, M.B. Kovac, S. Humphray, H.J. Thomas, E. Maher, G. Evans, A. Lucassen, C. Cummings, M. Stevens, L. Walker, D. Halliday, R. Armstrong, J. Paterson, S. Hodgson, T. Homfray, L. Side, L. Izatt, A. Donaldson, S. Tomkins, P. Morrison, S. Goodman, C. Brewer, A. Henderson, R. Davidson, V. Murday, J. Cook, N. Haites, T. Bishop, E. Sheridan, A. Green, C. Marks, S. Carpenter, M. Broughton, L. Greenhalge, M. Suri, P.C. Donnelly, J. Bell, D. Bentley, G. McVean, P. Ratcliffe, J. Taylor, A. Wilkie, J. Broxholme, D. Buck, R. Cornall, L. Gregory, J. Knight, G. Lunter, I. Tomlinson, D.L. Buck, Z. Kingsbury, G.L. McVean, P. Donnelly, R. Grocock, E. Hatton, C.C. Holmes, L. Hughes, P. Humburg, A. Kanapin, L. Murray, A. Rimmer, C. Petridis, R. Roylance, E.J. Sawyer, D.J. Kerr, S. Clark, J. Grimes, S.E. Kearsey, and R.S. Houlston. 2012. Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas. Nat Genet.
Pai, C.C., J. Schnick, S.A. MacNeill, and S.E. Kearsey. 2012. Conditional inactivation of replication proteins in fission yeast using hormone-binding domains. Methods. 57:227-233.
Havens, C.G., N. Shobnam, E. Guarino, R.C. Centore, L. Zou, S.E. Kearsey, and J.C. Walter. 2012. Direct role for proliferating cell nuclear antigen in substrate recognition by the E3 ubiquitin ligase CRL4Cdt2. J Biol Chem. 287:11410-11421.
2011
Hua, H., and S.E. Kearsey. 2011. Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2'-deoxyuridine. Nucleic Acids Res. 39:e60.
Guarino, E., M.E. Shepherd, I. Salguero, H. Hua, R.S. Deegan, and S.E. Kearsey. 2011. Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation. Nucleic Acids Res. 39:5978-5990.
2010
Wen, W.L., A.L. Stevenson, C.Y. Wang, H.J. Chen, S.E. Kearsey, C.J. Norbury, S. Watt, J. Bahler, and S.W. Wang. 2010. Vgl1, a multi-KH domain protein, is a novel component of the fission yeast stress granules required for cell survival under thermal stress. Nucleic Acids Res. 38:6555-6566.
Moss, J., H. Tinline-Purvis, C.A. Walker, L.K. Folkes, M.R. Stratford, J. Hayles, K.L. Hoe, D.U. Kim, H.O. Park, S.E. Kearsey, O. Fleck, C. Holmberg, O. Nielsen, and T.C. Humphrey. 2010. Break-induced ATR and Ddb1-Cul4(Cdt)(2) ubiquitin ligase-dependent nucleotide synthesis promotes homologous recombination repair in fission yeast. Genes Dev. 24:2705-2716.
2009
Pai, C.C., I. Garcia, S.W. Wang, S. Cotterill, S.A. Macneill, and S.E. Kearsey. 2009. GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast. Mol Biol Cell. 20:1213-1222.
Kearsey, S.E., and J. Gregan. 2009. Using the DHFR heat-inducible degron for protein inactivation in Schizosaccharomyces pombe. Methods Mol Biol. 521:483-492.
Cotterill, S., and S.E. Kearsey. 2009. DNAReplication: a database of information and resources for the eukaryotic DNA replication community. Nucleic Acids Res. 37:D837-839.
2008
Wang, S.W., A.L. Stevenson, S.E. Kearsey, S. Watt, and J. Bahler. 2008. Global role for polyadenylation-assisted nuclear RNA degradation in posttranscriptional gene silencing. Mol Cell Biol. 28:656-665.
Boe, C.A., I. Garcia, C.C. Pai, J.R. Sharom, H.C. Skjolberg, E. Boye, S. Kearsey, S.A. Macneill, M.D. Tyers, and B. Grallert. 2008. Rapid regulation of protein activity in fission yeast. BMC Cell Biol. 9:23.
2007
Tvegard, T., H. Soltani, H.C. Skjolberg, M. Krohn, E.A. Nilssen, S.E. Kearsey, B. Grallert, and E. Boye. 2007. A novel checkpoint mechanism regulating the G1/S transition. Genes Dev. 21:649-654.
Kearsey, S.E., A.L. Stevenson, T. Toda, and S.W. Wang. 2007. Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase. Mol Cell Biol. 27:1558-1567.
2006
Gregan, J., C.G. Riedel, A.L. Pidoux, Y. Katou, C. Rumpf, A. Schleiffer, S.E. Kearsey, K. Shirahige, R.C. Allshire, and K. Nasmyth. 2007. The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation. Curr Biol. 17:1190-1200.
Ralph, E., E. Boye, and S.E. Kearsey. 2006. DNA damage induces Cdt1 proteolysis in fission yeast through a pathway dependent on Cdt2 and Ddb1. EMBO Rep. 7:1134-1139.
Namdar, M., and S.E. Kearsey. 2006. Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast. Exp Cell Res. 312:3360-3369.